Demo unit available, please contact us: Tech@ScienceServices.de
NanoEnTeks ADAM-MC automatic cell counter measures total cell numbers and cell viabilities through cutting-edge detection technologies. Instead of tryphan blue staining which can lead to inaccurate data, ADAM utilizes sensitive fluorescence dye staining (PI), LED optics and CCD detection technologies to make the cell analysis more accurate and reliable.
Combined with a disposable microchip, the operation of the Automatic Cell Counter is now extremely simple, safe and cost-effective. The need for routine calibration of the device is completely eliminated. Cleaning and warm-up time is no longer necessary with ADAM. The disposable chip greatly reduces the risk of the exposure to hazardous samples, such as patients blood samples, virus infected cells, etc.
New technology behind the counting accuracy of ADAM-MC
The ADAM-MC automatic cell counter is a precision microscope integrated with fluorescence optic and image analysis software. After the samples are stained with a fluorescent dye, propium iodide, which intercalates DNA to stain the nucleus of target cells, fluorescent images are taken automatically. The system takes the 22-60 images by the movement of an automatic X-Y stage. The obtained images are processed by image analysis software integrated inside the system. All procedures are fully automatic once the stained samples have been dropped to the disposable chip and the chip is loaded.
- LED excitation
- Precision automatic stage
- Automatic focusing
- Sensitive CCD detection
- Automatic image analysis
- Disposable microchips
- Large counting volume guarantees accurate results
Conventional automatic cell counters utilize flow cells or counting chambers, which are permanently integrated inside the device. During or after cell counting, tubings or counting chambers are easily clogged by cell debris. For this reason, for conventional automatic cell counters, routine maintenance with a cleansing process is necessary.
The ADAM-MC automatic cell counter utilizes a precision disposable microchip to eliminate the problems of permanent counting chambers. Each microchip is designed for single use. Pumping, cleansing, and waste bottles are not necessary for the ADAM cell counter. Also, the risk of exposure to potential hazardous materials, such as patients blood samples, is completely eliminated with the disposable chips. Each microchip is produced by NanoEnTeks state-of-the-art manufacturing facility and validated for accuracy.
Repeatability & Accuracy
Graph 1: Correlation of total cell counting between hemocytometer and ADAM-MC using CHO cells.
Graph 2: Samples with low, medium and high concentrations of cells counted with ADAM-MC.
Graph 3: Comparison of cell viabilities between ADAM-MC and flow cytometry. SK-OV, HeLa and NCl-H23 cells were treated with 100, 300µM H2O2 for 3 hours, then analyzed by ADAM and flow cytometry. Values are given as means +-SD of three experiments.
1.What’s the filter range of ADAM-MC?
Excitation filter 500-550nm Emission filter 580-700nm
2.What’s the detection range of ADAM-MC?
Cell detection range is 1 x 104 - 1 x 107 cells/ml. The range between 5 x 104 - 4 x 106 cells/ml ADAM shows the most accurate data.
3. What’s the cell size that can be measured with ADAM-MC?
Cell diameters between 3 μm - 50 μm.
4. Can I use ADAM-MC just for mammalian cells?
Basically yes, but if fluorescence of target sample is within its filter range, it might be possible to use ADAM-MC for counting that samples. There are several reports that users count particles with ADAM-MC
Until now, cell counting and viability measurement for many types of cells have been performed manually using a hemocytometer with Trypan Blue exclusion to distinguish viable cells from non-viable cells. One drawback of this method, however, is the propensity for the staining of artifacts; another drawback is that the naked eye can only differentiate between cells in a limited concentration range in the hemocytometer chamber. This combined with the potential problem of cell aggregation and limited sample volume leads to the common variation of counts normally associated with this method. To address these problems, Digital Bio has developed the ADAM, which is based on a fluorescent microscopy technique for counting cells. The ADAM-MC utilizes sensitive fluorescence dye staining, LED optics and CCD detection technologies to make the cell analysis more accurate and reliable. To count cells using ADAM, the cells are mixed with a Propidium Iodide (PI) stain and directly pipetted on to a disposable plastic chip. The chip is then loaded onto a precision stage. The ADAM-MC system automatically focuses onto the chip and cells that have been stained are recorded by a sensitive CCD camera. The image results are automatically processed generating the cell count which is displayed on the front of the instrument. Simple. Fast. Accurate. Reliable.
ADAM-MC is based on staining mammalian cell DNA with a fluorescent dye, Propidium Iodide (PI). PI does not enter cells with intact cell membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. ADAM-MC provides two kinds of staining solutions. AccuStain Solution T for the total cell counting is composed of the fluorescent dye (PI) and lysis solution. AccuStain Solution N for the non-viable cell counting is composed of the fluorescent dye and PBS. In order to measure the total concentration of cells, the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI. The process of disrupting and staining is achieved by treatment with AccuStain Solution T. In the second solution, live cells remain intact and are not stained. Only the non-viable cells are stained and detected. After treatment, the prepared cells will be loaded into the chip. The viability will be automatically calculated in the ADAM-MC software after each measurement of the total cells and the non-viable cells
Summary of Advantages
- Ergonomic design
- Small and compact
- Plug and play technology
- Easy to operate
- External monitor to view the cell images
- Accurate and reliable results
- Less risk of exposure to hazardous samples