BrightSlide™ D

Fluorescence Enhancing Slide

For many applications an increased sensitivity compared to existing glass slides is required. The BrightSlide™ technology uses a proprietary dielectric coating to significantly enhance the signal intensity of fluorescent molecules from the surface. It can be read out with almost any system that illuminates and detects from the top. The technology makes use of only optical principles and substitutes conventional glass substrates.

The BrightSlide™ D (dielectric) provides a bare SiO2 surface.

Benefits

• Better signal-to-noise ratio
• Higher sensitivity
• Less sample material can be used
• More genes can be detected
• Existing instrument platform can be used
• Purely optical amplification of fluorescence signal
• Dielectric, high quality SiO2 surface ready to apply a biofunctional coating
  (various biofunctional coatings can be provided on request)

Applications

• Universal detection platform for fluorescence detection on planar surfaces, e.g. fluorescence detection of ultra thin samples
• Differential gene expression using Cy3 and Cy5 labels simultaneously
• Gene expression using Cy3 or Cy5 labels independently

Technical Data

• Substrate material: D263T eco, other materials on request
• Substrate dimensions: 75mm x 25mm x 1.1mm, other dimensions on request
• Active area: 63mm x 22mm
• Fluorescence enhancement optimized for one to three customer
  specific dyes on one slide
• Both polarizations (s- and p-polarization) can be used for excitation and emission
• 0° - 3° angle of incidence for excitation light
• Ultralow fluorescence background
• Ultraclean SiO2 surface
• Laser scribed, durable and chemically resistant label with a
  unique serial number or bar code
• Suitable for most of commercially available fluorescence microscopes
  with illumination and detection from the top
• Stringent batch to batch reproducibility
• Packed under clean-room (class 100) conditions

Enhancement of the fluorescence on BrightSlide™ Image of
150nm thick section of biological material using BrightSlide™


Fluorescence signal on standard glass slide Image of
150nm thick section of biological material using standard glass slide