EMS Glycerol Mount mit DABCO™

EMS Glycerol mit dem Antifade DABCO™ (1,4-Diazobicyclo-[2,2,2-octan]), ist ein wässriges Eindeckmedium für fluoreszentes Gewebe und Zellabstriche. Diese einzigartige Zusammensetzung verhindert das schnelle Ausbleichen von FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), Tetramethylrhodamine und Redox. Durch Lagerung der Proben bei 4°C im Dunkeln bleibt die Fluoreszenz erhalten. Nicht geeignet für Phycoerythrin (PE), Phycocyanin (PC) and Allophycocyanin (APC).

Instruction for use
Dehydration of specimen is not required.

1. Bring the vial to room temperature.
2. Turn the vial upside down and open the dropper to remove any air bubbles.
3. Apply 1-2 drops of mounting medium directly; cover slip carefully avoiding air bubbles.
4. The specimen is ready for visualization under a microscope.
5. One can seal the edges of cover slip with nail polish or any organic medium.
6. Method for applying Coverslip: Put 1-2 drops of Mounting medium on the specimen. After 3-4 minutes apply coverslip carefully avoiding air bubbles. Put Kimwipes on the top of coverslip, press gently to remove excess mounting medium. With 200 micropipette add organic mounting medium to seal the edges.
7. For storage it is recommended that the slide be stored in the dark at 2-8°C.