EMS Shield Mount mit DABCO™

EMS Shield Mount mit dem Antifade DABCO™ (1,4-Diazobicyclo-[2,2,2-octan]) ist ein wässriges Eindeckmedium mit geringer Viskosität zur temporären Eindeckung von Immunfluoreszenz-Proben. Diese einzigartige Zusammensetzung verhindert das schnelle Ausbleichen von FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), Tetramethylrhodamine und Redox. Durch Lagerung der Proben bei 4°C im Dunkeln bleibt die Fluoreszenz erhalten. Dieses Eindeckmedium enthält kein Phenylendiamin, welches die Immunfluoreszenz von Cy Farbstoffen zerstört.

Instructions for use

1. Bring the vial to room temperature.
2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.
3. Turn the vial upside down and open the dropper to remove any air bubbles.
4. Apply 2-3 drops of mounting medium and apply coverslip carefully without getting air bubbles.
5. The specimen is ready for visualization under a microscope.
6. The coverslip may move, so it may be necessary to seal the edges of cover slip with nail polish, any organic medium or the Limonene mounting medium (E17987-01).
7. Store slide at 2-8°C in the dark.

Handling and storage
2-8°C, Protect from light, DO NOT FREEZE.

Removal of coverslip
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deionized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.