Fluoro-Gel III mit PI, Eindeckmedium

Fluoro-Gel III ist ähnlich wie Fluoro-Gel, jedoch mit dem Zusatz von PI (Propidiumiodid) zur Gegenfärbung von DNA/RNA. Fluoro-Gel III verhindert das schnelle Ausbleichen von FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent protein (GFP), Tetramethylrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC) und Allophycocyanin (APC). Die Lagerung der Fluoreszenzproben sollte bei 4°C im Dunkeln erfolgen. Dieses Medium enthält kein Phenylendiamin, welches die Immunfluoreszenz von Cy Farbstoffen, RP-E, PC und APC zerstört. PI wird bei 535nm angeregt und emittiert bei 615nm, erzeugt eine rote Fluoreszenz.

Instructions for use

1. Bring the vial to room temperature.
2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light.
3. Turn the vial upside down and remove any air bubbles.
4. Apply 3-4 drops directly of mounting medium on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip, making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide on paper towel.
5. Let stand at room temperature for about 5 minutes.
6. Apply cover slip, carefully avoiding air bubbles.
7. The specimen is ready for visualization under microscope.
8. One can seal the edges of cover slip with nail polish or any organic medium or our Limonene mounting medium (cat.  E17987-01). If coverslip is not sealed air bubbles will appear in few days.
9. For long term storage it is recommended that the slide be stored in the dark at 2-8°C.

Handling and storage
2-8°C, Protect from light, DO NOT FREEZE.

Removal of coverslip
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deionized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.