General incubation protocol

1. Low Molecular Weight Block Step

2. High Molecular Weight Block Step
(using AURION Blocking Solutions)

3. Wash step
(all wash steps use the recommended incubation solution, containing AURION BSA-c™)

4. Primary antibody incubation
(in recommended incubation solution, containing AURION BSA-c™)

5. Wash steps
(see first wash step)

6. Immuno Gold conjugate incubation
(using AURION Ultra Small Immuno Gold Reagents or AURION Conventional Reagents)

7. Wash steps
(see first wash step)

8. Further wash steps
(see first wash step)

9. Silver Enhancement
(for LM: AURION R-Gent SE-LM / for EM: AURION R-Gent SE-EM)

10. Wash steps
(see first wash step)

11. Contrasting or Staining