Hematoxylin Staining Tips

Staining Tips With Hematoxylin

1. Check a slide from each batch of slides during times the workload is heavy to ensure good staining by the Hematoxylin.
2. Do not allow the slides to dry out at any point during your staining procedure.
3. Increase your staining time in Hematoxylin for tissues that have been in fixative longer than normal.
4. Increase your staining time by at least 1⁄3 for autopsy tissues.
5. Do not agitate the slides if you are using Ammonia water to Blue. The tissues are more susceptible to washing off if you agitate the slides.
6. Check the pH of the Hematoxylin daily.