UranyLess: FAQs
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UranyLess is a solution ready for use, a mix of lanthanides (rare-earths).
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In an aqueous solution (water).
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One year.
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Store it at room temperature away from direct sunlight.
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No, it is sold ready for use.
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UranyLess pH is 6.8-7.
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Simply drop UranyLess on your grid, and wait a minute. Dry, then contrast with lead citrate according to Reynolds method.
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Precipitates form when lead citrate is washed with cold water which is loaded with CO2. Use water that has never been at low temperature and never store lead citrate at low temperature.
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Yes it is - a double stain of UranyLess plus Lead citrate.
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Yes, it operates with every kind of resin (Epon, Araldite, Spurr).
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Yes, it can be used on negative staining.
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Some tests are in progress.
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No, because it is prepared in water. However, we are currently developing many formulations of UranyLess, including ethyl UranyLess and acetone UranyLess, the latter being the best-adapted to cryogenic use.
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Yes.
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We sell UranyLess in an airless 30ml bottle and also in a brown 200ml bottle.
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Its use is very simple; simply push on the head of the bottle to get a drop. When you release, the bottle back pump actuator lifts up. It prevents any air inlet in the bottle.
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It is a bottle in which air never enters. Some products, such as lead citrate, are atmospheric CO2 sensitive. Thanks to this system, those products have a longer shelf life. It also allows the product to be deposited drop by drop, quickly, cleanly and in any position.
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Yes, the 200ml bottle is available for use with automated staining equipment. When using UranyLess for automated staining, do not wash longer than 10 minutes or you run the risk of losing all contrast.
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