Tech Notes

Tech Notes für EM und LM Anwendungen (auch bekannt als “Kirsch Notes” von Electron Microscopy Sciences)

Sie möchten gerne wissen, wie man Formvar-befilmte Grids selbst herstellt oder warum Acetonitril eine gute Alternative für Propylenoxid ist?

Die Antworten auf diese und viele andere Fragen, sowie viele andere hilfreiche technische Tipps und
Produktinformationen für Anwendungen aus den Bereichen LM und EM finden Sie ab jetzt hier bei den “Tech Notes”.

(Mit freundlicher Genehmigung von Stacie Kirsch, EMS)

- General Lab and Safety Tips
  
  Latex Gloves - Allergy and Alternatives
  
  Increase Stability and Shelf Life of Chemicals
  
  Calculating Solutions and Dilutions (PDF)
  
  Providing Solutions: General Lab Safety (PDF)
- General Tips and Protocols for Sample Preparation for Microscopy (LM/EM)
  
  Preparation of Gelatin Adhesive
  
  Rapid Techniques for Specimen Preparation - REF
  
  Animal Tissue Techniques - Decalcification
  
  Paraffin and Plastic Techniques for Preparation of Bone Marrow Specimen
  
  The Mounting of Specimens for SEM with the Common Glue Gun
  
  Coverslip Treatment Test
  
  Quick Guide: Mircowave Processing of Biological Samples for EM (PDF)
- Light Microscopy and LM Specimen Handling
  
  Permanox Plastic; Non Fluorescent
  
  Poly-L-Lysine Coating of Slides
  
  Protocol: Köhler Alignment for Light Microscopes (PDF)
- Tissue Staining for Histology (LM)
  
  DEPEX - Is An Improved Mounting Medium
  
  Purification of Alcian Blue
  
  Hematoxylin Staining Tips
  
  A Methylene Blue-Azure II Stain
  
  A Toluidine Blue/Basic Fuchsin Stain for GMA Embedded Tissues
- Buffering, Fixation and Dehydration of Tissues (LM/EM)
  
  Use of Paraformaldehyde, Glutaraldehyde and Acrolein Buffer
  
  Paraformaldehyde Static Jumps
  
  Use of Acetonitrile
  
  Fixative for Use in Fixing Cornea
  
  Surgical Gloves - A Safety Tip -for usage with Glutaraldehyde
  
  Preparation of Karnovsky's Fixative (PDF)
  
  Providing Solutions: Buffers for Electron Microscopy (PDF)
  
  Providing Solutions: Dehydrants for Electron Microscopy (PDF)
  
  Providing Solutions: Fixatives for Electron Microscopy (PDF)
- Staining of Tissues with Heavy Metals (EM)
  
  Preparation of Osmium Tetroxide OsO4 Solution from Crystalline Form (PDF)
  
  Stability of Special Staining Solutions
  
  An Air-Tight Container for Small Amounts of Osmium Tetroxide
  
  Neutralization and Disposal of Osmium Tetroxide
- Post-Staining of Sections with Heavy Metals (EM)
  
  Lead Staining
  
  Common Lead Stock Solutions
  
  Handling of Lead Solutions
  
  Stable Lead Staining Solution
- Resin Embedding of Samples (EM)
  
  Providing Solutions: TEM Embedding Resins (PDF)
  
  Providing Solutions: Epoxy and Acrylic Resins (PDF)
  
  Embedding Samples in Epoxy and Acrylic Resins (PDF)
  
  Mixing Epoxy Resins (PDF)
  
  Preparation of Embedding Mixtures
  
  Epoxy Resins: Problems, Causes, and Solutions
  
  Embed812 - an Epon-812 Replacement
  
  Low Viscosity Kit Formation with ERL 4221
  
  Use of Accelerators for Epoxy Resins
  
  The Use of Flexibilizers and Plasticizers
  
  The Use of DMP-30 (2,2-Dimethoxy Propane)
  
  DMP-30 and DMAE VS BDMA
  
  Quetol and the Addition of Water
  
  Embedding Resin Stain Permability
  
  Storage of Epoxy Resins
  
  Storage of Embedding Resin Mixtures
  
  Reference: Re-Embedding of Tissue Poorly Embedded
  
  Reference: Re-Embedding of Paraffin-Embedded Specimen Tissue in Resin
  
  Adherence of LR White Sections to Glass Slides for Silver Enhancement Immugold Labeling
  
  LR White Technical Tips - Distinguishing LR White from Tissue
  
  LR White Technical Tips - Adjusting pH
  
  LR White Technical Tips - Improved Polymerization of Acrylic Resins
  
  Reference: Preparation of Glycol Methacrylate
  
  Reference: Flat Embedding with LR-White
- Ultramicrotomy and Sectioning Tips (LM/EM)
  
  Block Trimming for TEM (PDF)
  
  Thick Sectioning for TEM using Leica UC7 (PDF)
  
  Thick Sectioning for TEM using RMC PowerTome (PDF)
  
  Thin Sectioning for TEM (PDF)
  
  Flattening Semithin Sections
  
  Avoiding Contamination of Ultra-Thin Sections
  
  Dental Wax to Make Trufs
  
  Reference: Cryo Sectioning and the Use of Diamond Knives
  
  Elimination of Wrinkles in Semi-Thin Sections
- TEM Grid and Section Handling Tips (EM)
  
  Providing Solutions: Mouting of SEM Specimens (PDF)
  
  Mounting Powders, Granules and Fibers
- Mounting of SEM Samples (EM)
  
  Providing Solutions: Mouting of SEM Specimens (PDF)
  
  Mounting Powders, Granules and Fibers
- Critical Point Drying (EM)
  
  Critical Point Drying with the EMS 3100 (PDF)
  
  Critical Point Drying with the EMS Q850 (PDF)
  
  Processing of Biological Samples with HMDS for SEM (PDF)
- Cryofixation of Tissues (EM)
  
  Preservation of Phospholipids
  
  Cryo-Protectants
- Immunolabeling (LM/EM)
  
  The Use of Polyethylene Glycol
  
  AURION BLOCK 1
  
  AURION BLOCK 2
- EM Imaging and Analysis
  
  Providing Solutions: TEM Imaging Issues and Solutions (PDF)
  
  Guidelines: Qualitative X-ray Analysis (PDF)
  
  Protocol: Developing TEM Imaging Films (PDF)